IGFBP3 antibody (Biotin)

Catalog No. ABIN5690523

Product Details anti-IGFBP3 Antibody (Biotin)

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGFBP3 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP3. Human IGF-BP3 specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hIGF-BP3 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3.

Sandwich
To detect hIGF-BP3 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IGF-BP3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3.

Western Blot:
To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IGF-BP3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IGFBP3

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Alternative Name: IGF-BP3 (IGFBP3 Products)

Synonyms: BP-53 antibody, IBP3 antibody, AI649005 antibody, IGFBP-3 antibody, IGgfbp3 antibody, IGF-BP3 antibody, zgc:91788 antibody, IGFBP3 antibody, igfbp3 antibody, insulin like growth factor binding protein 3 antibody, insulin-like growth factor binding protein 3 antibody, IGFBP3 antibody, Igfbp3 antibody, igfbp3 antibody, IGFBP-3 antibody, LOC100305016 antibody

Background: Insulin like growth factor binding protein 3 (IGF-BP3) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGF-BP) and at least four low affinity binding proteins referred to as IGF-BP related proteins (IGFBPrP). The IGF-BP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The cDNA sequence encoding the mature human IGF-BP3 is fused to the signal peptide of CD33. Human IGF-BP3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGFI or IGFII and an acid labile subunit.

Gene ID: 3486

UniProt: P17936

Pathways: Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding