Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP5. Human IGF-BP5 specific antibody was purified by affinity chromatography and then biotinylated.
IGFBP5
Reactivity: Human
WB
Host: Rabbit
Polyclonal
RB54635
unconjugated
Application Notes
ELISA: Direct: To detect hIGF-BP5 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP5.
Sandwich To detect hIGF-BP5 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IGF-BP5 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP5.
Western Blot: To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Restrictions
For Research Use only
Format
Lyophilized
Storage
-20 °C
Storage Comment
IGF-BP5 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Target
IGFBP5
(Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))
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Background
IGF-BP5 is one of a family of proteins that bind to insulin-like growth factors (IGFs). IGFB-P5 is a multifunctional protein which acts not only as a traditional binding protein but also functions as a growth factor independent of IGFs to stimulate bone formation. Currently there are seven named IGF-BPs that form high affinity complexes with both IGFI and IGFII. IGF-BP5 is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGFI on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGF-BP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGF-BP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development.