WNT3A antibody (Biotin)

Catalog No. ABIN5690547

Product Details anti-WNT3A Antibody (Biotin)

Target: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNT3A antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-3a. Human WNT-3a specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hWNT-3a by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.

Sandwich
To detect hWNT-3a by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human WNT-3a as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.

Western Blot:
To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: WNT-3a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for WNT3A

Target: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Alternative Name: WNT-3a (WNT3A Products)

Synonyms: WNT3A antibody, Wnt-3a antibody, vt antibody, Zwnt[a] antibody, wnt3 antibody, wnt3 l antibody, wnt3l antibody, wnt[a] antibody, Xwnt-3a antibody, wnt-3a antibody, wnt3a-A antibody, xwnt3a antibody, WNT-3A antibody, WNT3 antibody, wingless-type MMTV integration site family, member 3A antibody, Wnt family member 3A antibody, wingless-type MMTV integration site family member 3A L homeolog antibody, WNT3A antibody, Wnt3a antibody, wnt3a antibody, wnt3a.L antibody

Background: Wnt3a is a secreted ligand for members of the Frizzled family of receptors. It is one of the closely-related Wnt family and has multiple roles in development and cell fate.

Gene ID: 89780

UniProt: P56704

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity