TNFSF13 antibody (AA 105-250)

Catalog No. ABIN5690647

Product Details anti-TNFSF13 Antibody

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Binding Specificity: AA 105-250

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TNFSF13 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purification: APRIL (monoclonal Sacha-2) antibody was purified from concentrated hybridoma tissue culture supernatant. APRIL (monoclonal Sacha-2) antibody was purified to 95% in SDS-PAGE.

Immunogen: APRIL (monoclonal Sacha-2) antibody was raised against recombinant human APRIL (aa. 105-250).

Clone: Sacha-2

Isotype: IgG2a

Application Details

Application Notes: APRIL antibody can be used to recognize human and mouse APRIL by ELISA (capture), Flow Cytometry, and Immunocytochemistry. Method: HEK-293T cells expressing surface membrane associated human APRIL (aa 92-233) or a mock control were injected in chicken liver and frozen in OCT. 8 μ,m sections of injected frozen liver were fixed in acetone, washed in PBS and incubated with Sacha-2 at 10 μ,g/mL for 1hr after blocking in PBS/1 % BSA. Human APRIL was revealed with 3-amino-9-Ethyl-carbazole (AEC) following 45 min. incubation with goat anti-rat HRP. Method: HEK 293T  cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mouse APRIL (thick line). Cells were incubated on ice for 30 min in 50 μ,l FACS buffer (PBS, 5 % Fetal calf serum, 0.02 % sodium azide) containing 1 μ,g/mL of Sacha-2 antibody.  After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry. This product is for research use only.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: APRIL antibody can be stored at 4°C, stable for one year.

Target Details for TNFSF13

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Alternative Name: APRIL (TNFSF13 Products)

Synonyms: APRIL antibody, CD256 antibody, TALL-2 antibody, TALL2 antibody, TRDL-1 antibody, ZTNF2 antibody, 2310026N09Rik antibody, April antibody, Tall2 antibody, Trdl1 antibody, SLAN antibody, ANP32B antibody, april antibody, tnf13 antibody, TNLG7B antibody, TNF superfamily member 13 antibody, tumor necrosis factor (ligand) superfamily, member 13 antibody, acidic leucine-rich nuclear phosphoprotein 32 family member B antibody, TNFSF13 antibody, Tnfsf13 antibody, LOC494442 antibody, tnfsf13 antibody

Gene ID: 8741, 9370

UniProt: Q15848

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response