ELISA: (direct and indirect: 1:2,000-1:10,000). Flow Cytometry: (1:100-1:500). Western Blot: (1:2,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05 % Tween 20 and 5 % skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Preservative
Without preservative
Storage
-20 °C
Storage Comment
Stable for at least 6 months after receipt when stored at -20°C.
DLK1 (Protein delta homolog 1, Preadipocyte factor 1, Pref-1) is a regulator of adipocyte differentiation found in serum and urine. DLK1 might be a decoy ligand. Serum or plasma levels of soluble DLK1 affect negatively or positively adipogenesis and control mesenchymal cell fate. DLK1 is frequently upregulated in myelodysplastic syndrome (MDS) patients compared to non-leukemic individuals.