CD90 antibody

Catalog No. ABIN5691126

Product Details anti-CD90 Antibody

Target: CD90 (THY1)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD90 antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Specificity: Thy-1 antiserum is directed against rat thymocyte-1 antigen (Thy-1) and also reacts with mouse Thy-1.

Immunogen: Thy-1 antibody is a rabbit polyclonal antiserum obtained from rabbits immunised with purified Thy-1 derived from rat brain.

Application Details

Application Notes: Thy-1 is suitable for the detection of rat and mouse Thy-1 by immunohistochemical staining of frozen tissue sections. After drying, the sections can be either left unfixed or fixed with acetone at 4 °C for 4 min or 95 % ethanol at 4 °C for 15 min or 4 % formaldehyde in phosphate buffered saline pH 7.4 at 20 °C for 15 min. Also isolated cells can be immunocytochemically stained by this antiserum. Optimal antibody dilutions for the different applications should be determined by titration. Recommended range 1:50 - 1:100.

Restrictions: For Research Use only

Handling

Buffer: Each vial contains 100 μL polyclonal antiserum containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C, or in small aliquots at -20°C.

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: Thy-1/CD90 (THY1 Products)

Synonyms: CD90 antibody, CD7 antibody, T25 antibody, Thy-1 antibody, Thy-1.2 antibody, Thy1.1 antibody, Thy1.2 antibody, Thy-1 cell surface antigen antibody, thymus cell antigen 1, theta antibody, THY1 antibody, Thy1 antibody

Background: Thymocyte-1 antigen (Thy-1), also known as CD90, is a glycophosphatidylinositol (GPI)-anchored cell surface glycoprotein that is a member of the immunoglobulin superfamily. Expression of Thy-1, originally identified as a marker of thymocyte differentiation in mice, has been found to vary between species. In rats, unlike mice, Thy-1 is expressed on bone marrow cells but not on peripheral T-cells. In both mice and rats, Thy-1 is abundantly expressed on thymocytes and neuronal cells and to a lesser extent on fibroblasts, myofibroblasts, vascular endothelial cells, mammary glands and other non-lymphoid tissues. In many tissues the level of Thy-1 expression changes during cell differentiation. The exact function of Thy-1 has not been fully determined but studies suggest that Thy-1 may be an important regulator of cell-cell and cell-matrix interactions due to its involvement in a range of biological processes including cell growth and differentiation, cell adhesion and migration, apoptotic signalling and tumour suppression.

UniProt: P01830, P01831

Pathways: Cell-Cell Junction Organization