BNIP3 antibody

Catalog No. ABIN5692799

Product Details anti-BNIP3 Antibody

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BNIP3 antibody is un-conjugated

Application: Western Blotting (WB)

Brand: Picoband™

Sequence: KEFLFKHPKR TATLSMRNTS VMKK

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for BNIP3 detection. Tested with WB in Human,Mouse,Rat.

Immunogen: A synthetic peptide corresponding to a sequence of human BNIP3 (KEFLFKHPKRTATLSMRNTSVMKK).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

References for anti-BNIP3 antibody (ABIN5692799)

Du, Li, Xu, Zhou, Zhang, Wang: "MicroRNA-145 induces apoptosis of glioma cells by targeting BNIP3 and Notch signaling." in: Oncotarget, Vol. 8, Issue 37, pp. 61510-61527, (2017) (PubMed).

Target Details for BNIP3

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Alternative Name: BNIP3 (BNIP3 Products)

Synonyms: NIP3 antibody, Nip3 antibody, BCL2 interacting protein 3 antibody, BCL2/adenovirus E1B interacting protein 3 antibody, BNIP3 antibody, Bnip3 antibody

Background:

Synonyms: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3, BNIP3, NIP3

Background: The Bcl-2 nineteen kilodalton interacting protein 3 (BNIP3 or NIP3), is a hypoxia-inducible proapoptotic member of the Bcl-2 family that induces cell death by associating with the mitochondria. BNIP3, expressed in skeletal muscle and in the brain at low levels, is primarily localized to the nucleus of glial cells of the normal human brain, as well as in the malignant glioma cell line U251. BNIP3 expression in the cytoplasm increases and localizes with the mitochondria, contributing to induction of cell death. Cellular protein BNIP3 interacts with E1B-19K, BCL-2, BCL-xL, and EBV-BHRF1. BNIP3 contains Bcl-2 homology 3 (BH3) domain and COOH-terminal transmembrane (TM) domain. The BH3 domain of BNIP3 mediates Bcl-2/Bcl-X(L) heterodimerization and confers pro-apoptotic activity, whereas the TM domain is critical for homodimerization, pro-apoptotic function, and mitochondrial targeting.

UniProt: Q12983

Pathways: Autophagy, Brown Fat Cell Differentiation