Galectin 10 antibody (AA 2-142)

Catalog No. ABIN5693147

Product Details anti-Galectin 10 Antibody

Target: Galectin 10 (CLC) (Charcot-Leyden Crystal Protein (CLC))

Binding Specificity: AA 2-142

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Galectin 10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Brand: Picoband™

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Galectin 10 detection. Tested with WB, Direct ELISA in Human.

Immunogen: E. coli-derived human Galectin 10 recombinant protein (Position: S2-R142).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Application Details: Western blot, 0.1-0.5 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Target Details for Galectin 10

Target: Galectin 10 (CLC) (Charcot-Leyden Crystal Protein (CLC))

Alternative Name: CLC (CLC Products)

Synonyms: GAL10 antibody, Gal-10 antibody, LGALS10 antibody, LGALS10A antibody, LPPL_HUMAN antibody, Clcp antibody, gal-10 antibody, Bsf3 antibody, CLC antibody, GAL 10 antibody, Gal-8 antibody, Gal8 antibody, Charcot-Leyden crystal galectin antibody, Charcot-Leyden crystal protein antibody, cardiotrophin-like cytokine factor 1 antibody, avian beta-defensin 10 antibody, CLC antibody, Clc antibody, Clcf1 antibody, AvBD10 antibody

Background:

Synonyms: Galectin-10, Gal-10, Charcot-Leyden crystal protein, CLC, Eosinophil lysophospholipase, Lysolecithin acylhydrolase, CLC, LGALS10, LGALS10A

Tissue Specificity: Expressed abundantly in the bone marrow. Expressed exclusively by eosinophils and basophils. Not detected in monocytes and neutrophils. Expressed in CD25-positive regulatory T-cells (Treg) (at protein level). Found in intestinal tissue from patients with Celiac disease, expression is directly related to the histological grade of mucosal damage and to the number of eosinophils found in the duodenal lesion (at protein level). Found in sputum of patients with eosinophilic inflammatory diseases such as asthma (at protein level).

Background: Eosinophil lysophospholipase is an enzyme that in humans is encoded by the CLC gene. Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene is a lysophospholipase expressed in eosinophils and basophils. It hydrolyzes lysophosphatidylcholine to glycerophosphocholine and a free fatty acid. This protein may possess carbohydrate or IgE-binding activities. It is both structurally and functionally related to the galectin family of beta-galactoside binding proteins. It may be associated with inflammation and some myeloid leukemias.

UniProt: Q05315

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response