Cullin 7 antibody

Catalog No. ABIN5697465

Product Details anti-Cullin 7 Antibody

Target: Cullin 7 (CUL7)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Immunogen: cullin 7

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab02081(CUL7 antibody) at dilution of 1:300

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for Cullin 7

Target: Cullin 7 (CUL7)

Alternative Name: CUL7 (CUL7 Products)

Synonyms: cullin-7 antibody, CUL7 antibody, Cullin-7 antibody, KIAA0076 antibody, dJ20C7.5 antibody, 2510004L20Rik antibody, AA409809 antibody, C230011P08Rik antibody, p185 antibody, p193 antibody, cullin 7 antibody, CUL7 antibody, Cul7 antibody

Background: Synonyms:KIAA0076 Background:Core component of the 3M and Cul7-RING(FBXW8) complexes, which mediates the ubiquitination of target proteins. Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer(PubMed:24793695). Interaction with CUL9 is required to inhibit CUL9 activity and ubiquitination of BIRC5(PubMed:24793696). Core component of a Cul7-RING ubiquitin-protein ligase with FBXW8, which mediates ubiquitination and consequent degradation of target proteins such as GORASP1, IRS1 and MAP4K1/HPK1(PubMed:21572988, PubMed:24362026). Ubiquitination of GORASP1 regulates Golgi morphogenesis and dendrite patterning in brain(PubMed:21572988). Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase(RPS6KB1 or RPS6KB2)(PubMed:18498745). The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradatation, thereby affecting cell proliferation and differentiation(PubMed:24362026). Acts as a regulator in trophoblast cell epithelial-mesenchymal transition and placental development(PubMed:20139075). Does not promote polyubiquitination and proteasomal degradation of p53/TP53(PubMed:16547496, PubMed:17332328). While the Cul7-RING(FBXW8) and the 3M complexes are associated and involved in common processes, CUL7 and the Cul7-RING(FBXW8) complex may be have additional functions.

Molecular Weight: 185 kDa

Gene ID: 9820

UniProt: Q14999

Pathways: ER-Nucleus Signaling