EPS8 antibody

Catalog No. ABIN5700102

Product Details anti-EPS8 Antibody

Target: EPS8 (Epidermal Growth Factor Receptor Pathway Substrate 8 (EPS8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPS8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Immunogen: epidermal growth factor receptor pathway substrate 8

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

human brain tissue were subjected to SDS PAGE followed by western blot with FNab02819(EPS8 antibody) at dilution of 1:500

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for EPS8

Target: EPS8 (Epidermal Growth Factor Receptor Pathway Substrate 8 (EPS8))

Alternative Name: EPS8 (EPS8 Products)

Synonyms: AW261790 antibody, MGC81285 antibody, MGC146320 antibody, zgc:56041 antibody, epidermal growth factor receptor pathway substrate 8 antibody, epidermal growth factor receptor pathway substrate 8 L homeolog antibody, EPS8 antibody, Eps8 antibody, eps8.L antibody, eps8 antibody

Background: Synonyms: Background:Signaling adapter that controls various cellular protrusions by regulating actin cytoskeleton dynamics and architecture. Depending on its association with other signal transducers, can regulate different processes. Together with SOS1 and ABI1, forms a trimeric complex that participates in transduction of signals from Ras to Rac by activating the Rac-specific guanine nucleotide exchange factor(GEF) activity. Acts as a direct regulator of actin dynamics by binding actin filaments and has both barbed-end actin filament capping and actin bundling activities depending on the context. Displays barbed-end actin capping activity when associated with ABI1, thereby regulating actin-based motility process: capping activity is auto-inhibited and inhibition is relieved upon ABI1 interaction. Also shows actin bundling activity when associated with BAIAP2, enhancing BAIAP2-dependent membrane extensions and promoting filopodial protrusions. Involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration and cancer cell migration and invasion. Acts as a regulator of axonal filopodia formation in neurons: in the absence of neurotrophic factors, negatively regulates axonal filopodia formation via actin-capping activity. In contrast, it is phosphorylated in the presence of BDNF leading to inhibition of its actin-capping activity and stimulation of filopodia formation. Component of a complex with DFNB31 and MYO15A that localizes at stereocilia tips and is required for elongation of the stereocilia actin core. Indirectly involved in cell cycle progression, its degradation following ubiquitination being required during G2 phase to promote cell shape changes.

Molecular Weight: 97 kDa

Gene ID: 2059

UniProt: Q12929

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization