UBE2O antibody

Catalog No. ABIN5700803

Product Details anti-UBE2O Antibody

Target: UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2O antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Immunogen: ubiquitin-conjugating enzyme E2O

Isotype: IgG

Application Details

Application Notes: WB : 1:200-1:2000

Comment:

HeLa cells were subjected to SDS PAGE followed by western blot with FNab09181(UBE2O antibody) at dilution of 1:500

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for UBE2O

Target: UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))

Alternative Name: UBE2O (UBE2O Products)

Synonyms: e2-230k antibody, E2-230K antibody, 9630022H21 antibody, B230113M03Rik antibody, mKIAA1734 antibody, RGD1310297 antibody, ubiquitin conjugating enzyme E2 O antibody, ubiquitin-conjugating enzyme E2O antibody, UBE2O antibody, ube2o antibody, Ube2o antibody

Background: Synonyms:E2-230K, FLJ12878, KIAA1734, UBE2O, Ubiquitin carrier protein O, Ubiquitin protein ligase O Background:E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins(PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity(PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis(PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal(NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location(PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASH1, leading to promote endosomal F-actin assembly(PubMed:23452853).

Molecular Weight: 18kDa

Gene ID: 63893

UniProt: Q9C0C9