MAGOH antibody

Catalog No. ABIN5701062

Product Details anti-MAGOH Antibody

Target: MAGOH (Mago-Nashi Homolog (MAGOH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAGOH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Immunogen: mago-nashi homolog, proliferation-associated (Drosophila)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

K-562 cells were subjected to SDS PAGE followed by western blot with FNab04954( MAGOH Antibody) at dilution of 1:1000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for MAGOH

Target: MAGOH (Mago-Nashi Homolog (MAGOH))

Alternative Name: MAGOH (MAGOH Products)

Synonyms: MAGOH1 antibody, MAGOHA antibody, Mago-m antibody, Mos2 antibody, MAGOH antibody, zgc:112220 antibody, mago antibody, xl-mago antibody, CG9401 antibody, Dm MGN antibody, DmMago antibody, Dmel\CG9401 antibody, MGN antibody, Mago antibody, Magoh antibody, l(2)57Ca antibody, mgn antibody, magoh antibody, mago homolog, exon junction complex core component antibody, protein mago nashi homolog antibody, mago homolog, exon junction complex core component L homeolog antibody, mago nashi antibody, MAGOH antibody, Magoh antibody, LOC736868 antibody, magoh antibody, magoh.L antibody, mago antibody, LOC106584057 antibody

Background: Synonyms:MAGOHA Background:Core component of the splicing-dependent multiprotein exon junction complex(EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay(NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X(and probably other apoptotic genes), specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S), the function is different from the established EJC assembly.

Molecular Weight: 17 kDa

Gene ID: 4116

UniProt: P61326