USP10 antibody

Catalog No. ABIN5701378

Product Details anti-USP10 Antibody

Target: USP10 (Ubiquitin Specific Peptidase 10 (USP10))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Immunogen: ubiquitin specific peptidase 10

Isotype: IgG

Application Details

Application Notes: WB : 1:500-1:5000,IP:1:200-1:2000,IF:1:10-1:100

Comment:

HeLa cells were subjected to SDS PAGE followed by western blot with FNab09304(USP10 antibody) at dilution of 1:500

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for USP10

Target: USP10 (Ubiquitin Specific Peptidase 10 (USP10))

Alternative Name: USP10 (USP10 Products)

Synonyms: T4F9.30 antibody, T4F9_30 antibody, ubiquitin-specific protease 9 antibody, ubpo antibody, USP10 antibody, id:ibd1190 antibody, wu:fc19h08 antibody, wu:fc76g05 antibody, wu:fi11g01 antibody, UBPO antibody, 2610014N07Rik antibody, Uchrp antibody, mKIAA0190 antibody, usp10-A antibody, ubiquitin specific peptidase 10 antibody, universal stress protein UspA antibody, ubiquitin-specific protease 9 antibody, ubiquitin carboxyl-terminal hydrolase 10 antibody, ubiquitin specific peptidase 10 S homeolog antibody, USP10 antibody, RR_RS14970 antibody, UBP9 antibody, PTRG_07201 antibody, MGYG_06403 antibody, usp10 antibody, Usp10 antibody, usp10.S antibody

Background: Synonyms:Deubiquitinating enzyme 10, KIAA0190, Ubiquitin thiolesterase 10, UBPO, USP10 Background:Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta(IL1B) stimulation or upon DNA damage(PubMed:25861989).

Molecular Weight: 90kDa

Gene ID: 9100

UniProt: Q14694