USP28 antibody
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- Target See all USP28 Antibodies
- USP28 (Ubiquitin Specific Peptidase 28 (USP28))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This USP28 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Immunogen
- ubiquitin specific peptidase 28
- Isotype
- IgG
- Top Product
- Discover our top product USP28 Primary Antibody
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- Application Notes
- WB : 1:500-1:5000,IP:1:200-1:2000,IHC:1:20-1:200
- Comment
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HeLa cells were subjected to SDS PAGE followed by western blot with FNab09322(USP28 antibody) at dilution of 1:1000
- Restrictions
- For Research Use only
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- Buffer
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze / thaw cycles.
- Storage
- -20 °C
- Expiry Date
- 12 months
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- Target
- USP28 (Ubiquitin Specific Peptidase 28 (USP28))
- Alternative Name
- USP28 (USP28 Products)
- Synonyms
- im:7138195 antibody, USP28 antibody, 9830148O20Rik antibody, AU022237 antibody, mKIAA1515 antibody, ubiquitin specific peptidase 28 antibody, USP28 antibody, usp28 antibody, Usp28 antibody
- Background
- Synonyms:Deubiquitinating enzyme 28, KIAA1515, Ubiquitin thiolesterase 28, USP28 Background:Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome(APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7(FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7(FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes(TSGs) in a subset of colorectal cancers(CRC) cells(PubMed:24623306).
- Molecular Weight
- 125kDa
- Gene ID
- 57646
- UniProt
- Q96RU2
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