NOL3 antibody

Catalog No. ABIN5704030

Product Details anti-NOL3 Antibody

Target: NOL3 (Nucleolar Protein 3 (Apoptosis Repressor with CARD Domain) (NOL3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Immunogen: nucleolar protein 3 (apoptosis repressor with CARD domain)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with FNab05784(NOL3 antibody) at dilution of 1:800

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for NOL3

Target: NOL3 (Nucleolar Protein 3 (Apoptosis Repressor with CARD Domain) (NOL3))

Alternative Name: NOL3 (NOL3 Products)

Synonyms: ARC antibody, FCM antibody, MYP antibody, NOP antibody, NOP30 antibody, B430311C09Rik antibody, MYC antibody, Nop30 antibody, Arc antibody, nucleolar protein 3 antibody, nucleolar protein 3 (apoptosis repressor with CARD domain) antibody, NOL3 antibody, Nol3 antibody

Background: Synonyms:ARC, Background:Isoform 1: May be involved in RNA splicing. Isoform 2: Functions as an apoptosis repressor that blocks multiple modes of cell death. Inhibits extrinsic apoptotic pathways through two different ways. Firstly by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex(DISC) assembly(By similarity). Secondly by interacting with CASP8 in a mitochondria localization-and phosphorylation-dependent manner, limiting the amount of soluble CASP8 available for DISC-mediated activation(By similarity). Inhibits intrinsic apoptotic pathway in response to a wide range of stresses, through its interaction with BAX resulting in BAX inactivation, preventing mitochondrial dysfunction and release of pro-apoptotic factors(PubMed:15004034). Inhibits calcium-mediated cell death by functionning as a cytosolic calcium buffer, dissociating its interaction with CASP8 and maintaining calcium homeostasis(PubMed:15509781). Negatively regulates oxidative stress-induced apoptosis by phosphorylation-dependent suppression of the mitochondria-mediated intrinsic pathway, by blocking CASP2 activation and BAX translocation(By similarity). Negatively regulates hypoxia-induced apoptosis in part by inhibiting the release of cytochrome c from mitochondria in a caspase-independent manner(By similarity). Also inhibits TNF-induced necrosis by preventing TNF-signaling pathway through TNFRSF1A interaction abrogating the recruitment of RIPK1 to complex I(By similarity). Finally through its role as apoptosis repressor, promotes vascular remodeling through inhibition of apoptosis and stimulation of proliferation, in response to hypoxia(By similarity). Inhibits too myoblast differentiation through caspase inhibition(By similarity).

Molecular Weight: 30-33 kDa

Gene ID: 8996

UniProt: O60936