FBXO18 antibody

Catalog No. ABIN5705793

Product Details anti-FBXO18 Antibody

Target: FBXO18 (F-Box Protein, Helicase, 18 (FBXO18))

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO18 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Immunogen: F-box protein, helicase, 18

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for FBXO18

Target: FBXO18 (F-Box Protein, Helicase, 18 (FBXO18))

Alternative Name: FBXO18 (FBXO18 Products)

Synonyms: FBH1 antibody, Fbx18 antibody, AU015756 antibody, Fbh1 antibody, F-box protein, helicase, 18 antibody, F-box protein 18 antibody, FBXO18 antibody, Fbxo18 antibody

Background: Synonyms:FBH1, FBX18 Background:3'-5' DNA helicase and substrate-recognition component of the SCF(FBXO18) E3 ubiquitin ligase complex that plays a key role in response to stalled/damaged replication forks(PubMed:11956208, PubMed:23393192). Involved in genome maintenance by acting as an anti-recombinogenic helicase and preventing extensive strand exchange during homologous recombination: promotes RAD51 filament dissolution from stalled forks, thereby inhibiting homologous recombination and preventing excessive recombination(PubMed:17724085, PubMed:19736316). Also promotes cell death and DNA double-strand breakage in response to replication stress: together with MUS81, promotes the endonucleolytic DNA cleavage following prolonged replication stress via its helicase activity, possibly to eliminate cells with excessive replication stress(PubMed:23319600, PubMed:23361013). Plays a major role in remodeling of stalled DNA forks by catalyzing fork regression, in which the fork reverses and the two nascent DNA strands anneal(PubMed:25772361). In addition to the helicase activity, also acts as the substrate-recognition component of the SCF(FBXO18) E3 ubiquitin ligase complex, a complex that mediates ubiquitination of RAD51, leading to regulate RAD51 subcellular location(PubMed:25585578).

Molecular Weight: 118-123 kDa

Gene ID: 84893

UniProt: Q8NFZ0