Histone H4 antibody (meArg3)

Catalog No. ABIN5706801

Product Details anti-Histone H4 Antibody

Target: Histone H4

Binding Specificity: meArg3

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)

Characteristics:

Synonyms: rabbit anti-Histone H4 monomethyl Arg3 antibody, H4R3me1, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4

Background: Histone H4 is an 11.4 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. Histone H4 shows transcriptional regulation that is both cell cycle-dependent and -independent. Histone H4 is modified by phosphorylation, acetylation, ribosylation, and methylation. Histone H4 has been shown to interact with other histone acetytransferases, histone deacetylases, and PARP. Anti-Histone H4 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Gene Name: HIST4H4

Purification: Anti-Histone H4 [Monomethyl Arg3] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat. Predicted to react with most mammal species. Cross-reactivity with Histone H4 from other sources has not been determined.

Immunogen:

Immunogen: Histone H4 [Monomethyl Arg3] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Arginine 3 of human Histone H4.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:50

Application Note: Anti-Histone H4 [Monomethyl Arg3] antibody is useful for Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunocytochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.43 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: 30 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details for Histone H4

Target: Histone H4

Abstract: Histone H4 Products

Synonyms: hist1h4l antibody, H1ft antibody, Hist4 antibody, Hist4h4 antibody, H4 antibody, X04652 antibody, FO108 antibody, H4/n antibody, H4F2 antibody, H4FN antibody, HIST2H4 antibody, H4C13 antibody, H4C7 antibody, F8N16.2 antibody, F8N16_2 antibody, histone H4 antibody, BcDNA:RH52884 antibody, CG3379 antibody, Dmel\CG3379 antibody, H4r antibody, His4-88CD antibody, His4R antibody, histone cluster 1, H4a L homeolog antibody, histone cluster 1 H4 family member B antibody, histone cluster 2, H4 antibody, histone cluster 2 H4 family member a antibody, histone H4 antibody, Histone H4 replacement antibody, Histone H4 antibody, hist1h4a.L antibody, Hist1h4b antibody, Hist2h4 antibody, HIST2H4A antibody, H4 antibody, H4C14 antibody, HIS4 antibody, HIST2H4 antibody, His4r antibody, his-38 antibody

Gene ID: 121504

NCBI Accession: NP_001029249

UniProt: P62805