TNFRSF1A antibody

Catalog No. ABIN5706820

Product Details anti-TNFRSF1A Antibody

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Reactivity: Human, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFRSF1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Characteristics:

Synonyms: TBP1 antibody, TNFR1 antibody, TNFR55 antibody, TNFRSF1A antibody, Tumor necrosis factor alpha receptor antibody, rabbit anti-Tumor Necrosis Factor p55 Antibody, rabbit anti-TNF p55 Antibody

Background: Tumor necrosis factor receptor superfamily member 1A (TNF p55 Receptor) is a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. It contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.

Gene Name: TNFR-I

Purification: This antiserum has been heated to 56°C for 30 minutes.  The antiserum is directed against the extracellular domain of the cell bound human TNFRp55.  In general, this antibody also detects primate sTNFRp55.  The antibody does not recognize human sTNFRp75.  In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the TNFRp55 found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid.

Immunogen:

Immunogen: The whole rabbit serum was prepared by repeated immunizations with recombinant human sTNFRp55 expressed in Chinese Hamster Cells.

Immunogen Type: Recombinant Protein

Application Details

Application Notes:

Application Note: This antiserum against Human TNF p55 Receptor (sTNFRp55) has been tested for use in ELISA,  radioimmunoassays, immunoblotting and immunoprecipitation.  Reactivity in other immunoassays is unknown.This product has been assayed for the ability to immunoprecipitate antigen.  A dilution range of 1:400 to 1:800 is suggested for this immunoassay.  For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.  This product has been assayed by immunoblot  using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate.  A working dilution range of 1:200 to 1:400 is suggested for this application.  This product has been assayed by ELISA against soluble (extracellular) sTNFRp55 using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature.  A working dilution range of 1:1,000 to 1:2,000 is suggested for this product.  For use in ELISA formats, this antibody is best used as the second antibody  in combination with a monoclonal antibody as a capture antibody.  This product has been assayed by radioimmunoassay against antigen.  A dilution of 1:8,000 is suggested for this immunoassay.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:400 to 1:800

ELISA Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details for TNFRSF1A

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Alternative Name: TNF p55 Receptor (TNFRSF1A Products)

Synonyms: CD120a antibody, FPF antibody, MS5 antibody, TBP1 antibody, TNF-R antibody, TNF-R-I antibody, TNF-R55 antibody, TNFAR antibody, TNFR1 antibody, TNFR1-d2 antibody, TNFR55 antibody, TNFR60 antibody, p55 antibody, p55-R antibody, p60 antibody, TNF-R1 antibody, TNF-alphaR1 antibody, TNFRI antibody, TNFRp55 antibody, TNFalpha-R1 antibody, Tnfr-2 antibody, Tnfr1 antibody, fpf antibody, tbp1 antibody, p55-r antibody, tnf-r antibody, tnfar antibody, tnfr1 antibody, cd120a antibody, tnfr55 antibody, tnfr60 antibody, xtnfr1 antibody, wu:fj65b10 antibody, zgc:85914 antibody, TNFR I antibody, TNFRSF1A antibody, TNFA antibody, cTNF antibody, TNF receptor superfamily member 1A antibody, tumor necrosis factor receptor superfamily, member 1a antibody, TNF receptor superfamily member 1A S homeolog antibody, tumor necrosis factor antibody, TNFRSF1A antibody, Tnfrsf1a antibody, tnfrsf1a.S antibody, tnfrsf1a antibody, TNF antibody

Gene ID: 7132

UniProt: P19438

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway