TNFRSF1A antibody

Catalog No. ABIN5706821

Product Details anti-TNFRSF1A Antibody

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFRSF1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Characteristics:

Synonyms: rabbit anti-TNFp55 Receptor Antibody, TBP1 antibody, TNFR1 antibody, TNFR55 antibody, TNFRSF1A antibody, Tumor necrosis factor alpha receptor antibody

Background: Human TNF p55 Receptor Antibody recognizes TNFR1, the p55 soluble TNFR protein. sTNFRp55 is one of the two transmembrane receptors for tumor necrosis factor (TNF) with different molecular weight, 55 kD (p55) and 75 kD (p75), have been identified. The extracellular part of both receptors are shedded by proteolytic cleavage and exist as soluble receptors which bind to TNF in plasma and other biological fluids. sTNFR proteins have been implicated in certain leukemias. Anti-sTNFRp55 antibody is suitable for researchers in Cancer, Immunology, and Cell Biology.

Gene Name: TNFR-I

Purification: Anti-Human TNF p55 Receptor Antibody is an IgG preparation of whole rabbit serum purified by Protein G chromatography.  This antibody is directed against the extracellular domain of the cell bound human TNFRp55.  In general, this antibody also detects primate sTNFRp55.  The antibody does not recognize human sTNFRp75.  In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the TNFRp55 found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid.  This antibody can be used for FACS analysis.  Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically.

Immunogen:

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human sTNFRp55 expressed in Chinese Hamster Cells.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-TNF p55 Receptor (sTNFRp55) antibody has been tested for use in ELISA,  radioimmunoassays, immunoblotting, flow cytometry and immunoprecipitation.  Reactivity in other immunoassays is unknown. This product has been assayed for the ability to immunoprecipitate antigen and a dilution range of 1:400 to 1:800 is suggested.  For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.  This product has been assayed by immunoblot and a working dilution range of 1:200 to 1:400 is suggested for this application.  This product has been assayed by ELISA against soluble (extracellular) sTNFRp55 at a dilution range of 1:2,000 to 1:8,000.  For use in ELISA formats, this antibody is best used as the second antibody  in combination with a monoclonal antibody as a capture antibody.  This product has been assayed by radioimmunoassay against antigen.  A dilution of 1:8,000 is suggested for this immunoassay.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details for TNFRSF1A

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Alternative Name: TNF p55 Receptor (TNFRSF1A Products)

Synonyms: CD120a antibody, FPF antibody, MS5 antibody, TBP1 antibody, TNF-R antibody, TNF-R-I antibody, TNF-R55 antibody, TNFAR antibody, TNFR1 antibody, TNFR1-d2 antibody, TNFR55 antibody, TNFR60 antibody, p55 antibody, p55-R antibody, p60 antibody, TNF-R1 antibody, TNF-alphaR1 antibody, TNFRI antibody, TNFRp55 antibody, TNFalpha-R1 antibody, Tnfr-2 antibody, Tnfr1 antibody, fpf antibody, tbp1 antibody, p55-r antibody, tnf-r antibody, tnfar antibody, tnfr1 antibody, cd120a antibody, tnfr55 antibody, tnfr60 antibody, xtnfr1 antibody, wu:fj65b10 antibody, zgc:85914 antibody, TNFR I antibody, TNFRSF1A antibody, TNFA antibody, cTNF antibody, TNF receptor superfamily member 1A antibody, tumor necrosis factor receptor superfamily, member 1a antibody, TNF receptor superfamily member 1A S homeolog antibody, tumor necrosis factor antibody, TNFRSF1A antibody, Tnfrsf1a antibody, tnfrsf1a.S antibody, tnfrsf1a antibody, TNF antibody

Gene ID: 7132

UniProt: P19438

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway