IL1R1 antibody

Catalog No. ABIN5706822

Supplier Product No.: 109-401-304-0100

The Rabbit Polyclonal anti-IL1R1 antibody (ABIN5706822) specifically detects IL1R1 in WB, IHC, ELISA, IP and FACS. The antibody is reactive with Human and Primate samples.

Quick Overview for IL1R1 antibody (ABIN5706822)

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Reactivity: Human, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1R1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-IL1R1 Antibody

Supplier Product No.: 109-401-304-0100

Supplier: Rockland

Purpose: IL-1 Receptor Type I Antibody

Cross-Reactivity (Details): The antiserum is directed against the extracellular domain of the human IL-1R type I, but precipitates the intact receptor expressed on human cells.

Characteristics: Synonyms: IL1RT1 antibody, Interleukin 1 receptor alpha type 1 antibody, Interleukin 1 receptor antagonist protein (IRAP) antibody, Antigen CD121a antibody, CD121A antibody, CD121a antigen antibody, D2S1473 antibody, IL 1 antibody, rabbit anti-IL-1 Receptor Type I Antibody, rabbit anti-interleukin-1 Receptor Type I Antibody

Purification: This antiserum has been heated to 56°C for 30 minutes.

Sterility: Sterile filtered

Immunogen:

Immunogen: IL-1 Receptor Type I Antibody was prepared by repeated immunizations with recombinant human IL-1R type I.

Immunogen Type: Recombinant Protein

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: User Optimized

Application Note: This antiserum against anti-Human IL-1 Receptor Type I (IL-1RI) has been tested in western blot. This antibody is suitable for use in ELISA, radioimmunoassays and immunoprecipitation.  Reactivity in other immunoassays is unknown. This product has been assayed for the ability to immunoprecipitate antigen.  A dilution range of 1:400 to 1:800 is suggested for this immunoassay.  For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.  This product has been assayed by ELISA against soluble (extracellular) IL-1RI using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature.  A working dilution range of 1:1,000 to 1:2000 is suggested for this product.  This product has been assayed by radioimmunoassay against antigen.  A dilution of 1:8,000 is suggested for this immunoassay. 

Neutralization Dilution: User Optimized

Western Blot Dilution: User Optimized

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 84 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store IL-1 Receptor Type I Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for IL1R1

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Alternative Name: IL1R1

Background: Background: IL-1 Receptor Type I is a cytokine receptor which binds interleukin 1. Anti-IL-1 Receptor Type I antibody is ideal for researchers interested in Immunology, Signal Transduction and Cancer.

Gene ID: 3554

UniProt: P14778

Pathways: NF-kappaB Signaling, Carbohydrate Homeostasis, Cancer Immune Checkpoints