TNF alpha antibody

Catalog No. ABIN5706833

Supplier Product No.: 110-401-321-0100

The Rabbit Polyclonal anti-TNF alpha antibody is suitable to detect TNF alpha in samples from Mouse. It has been validated for WB, IHC and ELISA.

Quick Overview for TNF alpha antibody (ABIN5706833)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-TNF alpha Antibody

Supplier Product No.: 110-401-321-0100

Supplier: Rockland

Purpose: TNF alpha Antibody

Cross-Reactivity (Details): The antiserum is directed against mature 17,000 MW mouse TNFa and is useful in determining its presence in various assays.

Characteristics: Synonyms: rabbit anti-Mouse TNF alpha Antibody, APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody

Purification: This antiserum has been heated to 56°C for 30 minutes.

Sterility: Sterile filtered

Immunogen:

Immunogen: The whole rabbit serum was prepared by repeated immunizations with recombinant mouse TNFa produced in E.coli.

Immunogen Type: Recombinant Protein

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100 - 1:500

Application Note: This antiserum against Mouse TNFa has been tested for use in immunoblotting.  This antibody is suitable for use in neutralizations, ELISA, radioimmunoassay, immunoprecipitation, and immunohistochemistry. Reactivity in other immunoassays is unknown. It recognizes the 17,000 MW TNFa. This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples.  This antibody is also useful for neutralization of mouse activity in bioassays.  It does not neutralize the biological activity of lymphotoxin.  For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG is recommended.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tnf

Background: Background: TNF alpha Antibody detects TNF-a protein. TNF-a is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is a potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Anti-TNF-alpha Antibody is ideal for investigators involved in Cell Signaling, Immunology and Signal Transduction research.

Gene ID: 21926

UniProt: P06804

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome