ATG4D antibody (Internal Region)

Catalog No. ABIN570736

Product Details anti-ATG4D Antibody

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ATG4D antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: ATG4D

Sequence: CSQLAQPTLR LPRT

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence CSQLAQPTLRLPRT, from the internal region (near C terminus) of the protein sequence according to NP_116274.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Pancreas. Recommended concentration: 2.5 μg/mL.

Western Blot: Preliminary experiments gave an approx. 26 kDa band in Human Skeletal Muscle lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ATG4D

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Alternative Name: ATG4D (ATG4D Products)

Synonyms: APG4-D antibody, APG4D antibody, AUTL4 antibody, 9830134P12Rik antibody, Apg4d antibody, Atg4dl antibody, Autl4 antibody, apg4d antibody, autophagy related 4D cysteine peptidase antibody, autophagy related 4D, cysteine peptidase antibody, autophagy related 4D, cysteine peptidase L homeolog antibody, ATG4D antibody, Atg4d antibody, atg4d.L antibody

Background: ATG4D, ATG4 autophagy related 4 homolog D (S. cerevisiae), APG4-D, APG4D, AUTL4, APG4 autophagy 4 homolog D, AUT-like 4, cysteine endopeptidase, autophagin-4, autophagy-related cysteine endopeptidase 4, cysteine protease involved in autophagy

Gene ID: 84971, 235040, 686505

NCBI Accession: NP_116274

Pathways: Autophagy