Recombinant beta-2 Microglobulin antibody (full length)

Catalog No. ABIN5707815

The Rabbit Monoclonal anti-beta-2 Microglobulin antibody is suitable to detect beta-2 Microglobulin in samples from Human. It has been validated for WB, FACS, IF and IHC (p).

Quick Overview for Recombinant beta-2 Microglobulin antibody (full length) (ABIN5707815)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This beta-2 Microglobulin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: B2M-1857R

Product Details anti-beta-2 Microglobulin Antibody

Binding Specificity: full length

Purpose: Recombinant B2M Antibody / Beta-2 Microglobulin (azide and preservative free)

Purification: Protein A affinity chromatography

Immunogen: Full length recombinant human protein was used as the immunogen for the recombinant B2M antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the recombinant B2M antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant B2M antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: B2M

Background: Recognizes a protein of 12 kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 Molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 Molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 Molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process