Retinoblastoma Protein (Rb) antibody

Catalog No. ABIN5708009

This Rabbit Monoclonal antibody specifically detects Retinoblastoma Protein (Rb) in IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for Retinoblastoma Protein (Rb) antibody (ABIN5708009)

Target: Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Retinoblastoma Protein (Rb) antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB1-2313R

Product Details anti-Retinoblastoma Protein (Rb) Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: A human recombinant partial protein was used as immunogen for this recombinant RB1 antibody.

Isotype: IgG kappa

Application Details

Application Notes: Due to variation in protocol and secondary antibody used, the recombinant RB1 antibody may need to be titered for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistology (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: The recombinant RB1 antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Target Details for Retinoblastoma Protein (Rb)

Target: Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Alternative Name: Retinoblastoma / Rb

Background: Recognizes a 105  kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Pathways: Mitotic G1-G1/S Phases