RNF7 antibody (Internal Region)

Catalog No. ABIN571089

This anti-RNF7 antibody is a Goat Polyclonal antibody detecting RNF7 in ELISA, WB and IHC. Suitable for Human.

Quick Overview for RNF7 antibody (Internal Region) (ABIN571089)

Target: RNF7 (Ring Finger Protein 7 (RNF7))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RNF7 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-RNF7 Antibody

Binding Specificity: Internal Region

Purpose: RNF7

Sequence: DACLRCQAEN KQE

Specificity: This antibody is expected to recognize isoform 1 (NP_055060.1).

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DACLRCQAENKQE, from the internal region of the protein sequence according to NP_055060.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 11 kDa band observed in Human Human Heart lysates (calculated MW of 12.7 kDa according to NP_055060.1). Recommended concentration: 1-3 μg/mL. An additional band of 28 kDa was consistently observed, however this band was not blocked by th

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RNF7

Target: RNF7 (Ring Finger Protein 7 (RNF7))

Alternative Name: RNF7

Background: RNF7, ring finger protein 7, CKBBP1, ROC2, SAG, OTTHUMP00000180645, regulator of cullins 2, sensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2, zinc RING finger protein SAG

Gene ID: 9616, 19823

NCBI Accession: NP_055060

Pathways: Positive Regulation of Endopeptidase Activity