HLA-A+B+C+E antibody
Reactivity: Human
FACS, IHC (fro), WB
Host: Mouse
Monoclonal
TP25-99SF
unconjugated
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- Target
- HLA-A+B+C+E
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
- Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
- Specificity
- The mouse monoclonal antibody TP25.99SF recognizes an extracellular epitope on HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for discrimination between HLA-G and other HLA-class I antigens.
- Cross-Reactivity (Details)
- Human
- Purification
- Purified by protein-A affinity chromatography.
- Purity
- > 95 % (by SDS-PAGE)
- Immunogen
- IFN-gamma-treated human melanoma cells Colo 38
- Clone
- TP25-99SF
- Isotype
- IgG1
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anti-HLA-A+B+C+E antibody (PE)
Reactivity: Human FACS Host: Mouse Monoclonal TP25-99SF PE
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- Application Notes
- Flow cytometry: Recommended dilution: 1-4 μg/mL.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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- Target
- HLA-A+B+C+E
- Alternative Name
- HLA-ABCE
- Background
- HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g. HLA-E, HLA-G.
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