PUMA antibody (Internal Region)

Catalog No. ABIN571246

This Goat Polyclonal antibody specifically detects PUMA in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for PUMA antibody (Internal Region) (ABIN571246)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PUMA Antibody

Binding Specificity: Internal Region

Purpose: BBC3

Sequence: DGGRPQRTVR

Specificity: This antibody is expected to recognize isoform 1 (NP_001120712.1) only.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-DGGRPQRTVR

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 24 kDa band observed in Human peripheral blood mononucleocytes lysates (calculated MW of 26.5 kDa according to NP_001120712.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: BBC3

Background: BBC3, BCL2 binding component 3, JFY1, PUMA, Bcl-2 binding component 3

Gene ID: 27113

NCBI Accession: NP_001120712

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity