KIT antibody

Catalog No. ABIN571350

Cited in 7+ publications. The Mouse Monoclonal anti-KIT antibody (Clone 104D2) (ABIN571350) specifically detects KIT in FACS, IP, ICC and IHC (fro). The antibody is reactive with Human, Cow and Non-Human Primate samples.

Quick Overview for KIT antibody (ABIN571350)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivity: Human, Cow, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This KIT antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 104D2

Product Details anti-KIT Antibody

Purpose: Anti-Hu CD117 Purified

Cross-Reactivity (Details): Human, Non-Human Primates, Bovine

Characteristics: The mouse monoclonal antibody 104D2 detects extracellular part of CD117 / c-Kit protooncogen.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: MOLM-1 megakaryocytic cells

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-KIT antibody (ABIN571350)

Stevenson, McGlynn, Hodge, McLinden, George, Davies, Shiels: "Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations." in: Stem cells and development, Vol. 18, Issue 10, pp. 1389-98, (2009) (PubMed).

Wihlidal, Varga, Pfeilstöcker, Karlic: "Expression and functional significance of osteocalcin splicing in disease progression of hematological malignancies." in: Leukemia research, Vol. 30, Issue 10, pp. 1241-8, (2006) (PubMed).

Blair, Sutherland: "Primitive acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo lack surface expression of c-kit (CD117)." in: Experimental hematology, Vol. 28, Issue 6, pp. 660-71, (2000) (PubMed).

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, (2000) (PubMed).

Broudy, Lin, Liles, Corey, OLaughlin, Mou, Linnekin: "Signaling via Src family kinases is required for normal internalization of the receptor c-Kit." in: Blood, Vol. 94, Issue 6, pp. 1979-86, (1999) (PubMed).

Broudy, Lin, Bühring, Komatsu, Kavanagh: "Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer." in: Blood, Vol. 91, Issue 3, pp. 898-906, (1998) (PubMed).

Rappold, Ziegler, Köhler, Marchetto, Rosnet, Birnbaum, Simmons, Zannettino, Hill, Neu, Knapp, Alitalo, Alitalo, Ullrich, Kanz, Bühring: "Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase." in: Blood, Vol. 90, Issue 1, pp. 111-25, (1997) (PubMed).

Target Details for KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: CD117

Background: KIT proto-oncogene, receptor tyrosine kinase,CD117 / c-Kit (stem cell factor receptor) is a 145 kDa receptor tyrosine kinase that regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. Mutations of CD117 / c-Kit can lead to growth and progression of tumours. After binding of its ligand, SCF (stem cell factor), CD117 / c-Kit is autophosphorylated on its intracellular domains and activated. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells and various cancer cells, e.g. acute myeloid leukemia cells.,c-Kit, PBT, SCFR

Gene ID: 3815

UniProt: P10721

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity