Glutathione antibody (DyLight 488)
FACS, IF
Host: Mouse
Monoclonal
L4H
DyLight 488
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- Target See all Glutathione Antibodies
- Glutathione
- Reactivity
- Please inquire
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Glutathione antibody is conjugated to DyLight 488
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF)
- Brand
- AbX™
- Characteristics
- Designed for Immunofluorescence and FACS Analysis Supplied as 50 µg in PBS For use under Non-Reducing conditions
- Purification
- Purified
- Immunogen
- Glutathione conjugated to Keyhole Limpet Hemocyanin
- Clone
- L4H
- Isotype
- IgG2a
- Top Product
- Discover our top product Glutathione Primary Antibody
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anti-Glutathione antibody
WB, IP, IA Host: Mouse Monoclonal L4H unconjugated
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- Application Notes
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1:5-1:50 IMMuNOFLuOresCeNCe Jurkat cells, permeabilized and incubated with our DyLight® 488 labeled Glutathione monoclo- nal, A001F-100UL, clone L4H, at a 1:25 dilution. 488 Filter unfiltered
Flow analysis of Jurkat cells processed for immunofluorescence as above. Unstained cells Stained at 1x Stained at 5x - Comment
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Designed for Immunofluorescence and FACS Analysis Supplied as 50 μg in PBS For use under Non-Reducing conditions
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 500 μg/mL
- Buffer
- 500 μg/mL in Phosphate Buffered Saline at pH 7.2 containing 0.09 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Storage
- 4 °C
- Storage Comment
- Short Term: 4°C. Extended: Aliquot and freeze at -20°C
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- Target
- Glutathione
- Abstract
- Glutathione Products
- Synonyms
- GT antibody, ILBP antibody, ILLBP antibody, PIP antibody, I-15P antibody, I-BABP antibody, ILBP3 antibody, Illbp antibody, I-BALB antibody, I-BAP antibody, fatty acid binding protein 6 antibody, fatty acid binding protein 6, ileal (gastrotropin) antibody, FABP6 antibody, Fabp6 antibody
- Target Type
- Chemical
- Background
- Glutathione (GSH) is the highest concentration non-protein thiol in mammalian cells and is present in concentrations of 0.5 to 10 mM. GSH plays a key role in many biological processes, including the synthesis of proteins and DNA, the transport of amino acids, and the protection of cells against oxidation. Harmful hydrogen peroxide cellular levels are minimized by the enzyme glutathione peroxidase (GP) using GSH as a reductant. The oxidized GSH dimer, GSSG, is formed from GSH and peroxide by the GP reaction. An important role of GSSG in the NFkB activating signal cascade is suggested by the fact that the potent NFkB inducer TPA increases intracellular GSSG levels and GSSG/GSH ratios. The AbX™ DyLight® 488-labeled dye is similar to Alexa 488, Cy2 or FITC. It will measure GSH-protein complexes under non-reducing conditions.
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