HDAC5/9 (pSer220), (pSer259) antibody

Catalog No. ABIN5956536

Product Details

Target: HDAC5/9

Binding Specificity: pSer220, pSer259

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: HDAC5,KIAA0600,Histone deacetylase 5,HD5,Antigen NY-CO-9,HDAC9,HDAC7,HDAC7B,HDRP,KIAA0744,MITR,Histone deacetylase 9,HD9,Histone deacetylase 7B,HD7,HD7b,Histone deacetylase-related protein,MEF2-interacting transcription rep

Purification: Affinity purification

Immunogen: Synthesized peptide derived from human HDAC5/9 around the phosphorylation site of S259/220.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:10000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide,0.5 % BSA and 50 % glycerol  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details

Target: HDAC5/9

Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.HDAC5 (Histone Deacetylase 5) is a Protein Coding gene. Diseases associated with HDAC5 include Polycystic Kidney Disease, Adult Type I. Among its related pathways are PEDF Induced Signaling and Phospholipase-C Pathway. GO annotations related to this gene include transcription factor binding and transcription corepressor activity. An important paralog of this gene is HDAC4. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC9 has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.HDAC9 (Histone Deacetylase 9) is a Protein Coding gene. Diseases associated with HDAC9 include Gastrointestinal Neuroendocrine Tumor and Cutaneous T Cell Lymphoma. Among its related pathways are PEDF Induced Signaling and Phospholipase-C Pathway. GO annotations related to this gene include transcription factor binding and histone deacetylase binding. An important paralog of this gene is HDAC5.

Molecular Weight: 122kDa

Gene ID: 10014, 9734