His Tag antibody

Catalog No. ABIN595727

The Mouse Monoclonal anti-His Tag antibody is suitable to detect His Tag. It has been validated for WB, IP and IF.

Quick Overview for His Tag antibody (ABIN595727)

Target: His Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-His Tag Antibody

Specificity: Recognizes His-tagged tagged proteins overexpressed in cells, including both amino- or carboxy-termini of targeted proteins in transfected mammalian cells. Species cross-reactivity: human, mouse, rat, others.

Purification: Purified

Immunogen: 6x His synthetic peptide.

Type of Immunogen: Synthetic peptide

Isotype: IgG2b

Application Details

Application Notes: Approved: IF, IP (1:200 - 1:400), WB (1:1000 - 1:4000)

Usage: Suitable for use in Western Blot, Immunoprecipitation, and Immunofluorescence. Western Blot: 1:1000-1:4000. Immunoprecipitation: 1:200-1:400.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for His Tag

Target: His Tag

Target Type: Tag