Topo IIalpha antibody

Catalog No. ABIN5962665

Product Details

Target: Topo IIalpha

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: Alpha isozyme,ATP hydrolyzing DNA topoisomerase II alfa,DNA gyrase,DNA topoisomerase (ATP hydrolyzing),DNA topoisomerase 2 alpha,DNA topoisomerase 2-alpha,DNA topoisomerase II 170 kD,DNA topoisomerase II alpha isozyme,DNA topoisomerase II,DNA Topoisomerase2,TOP 2A,TOP2,TOP2A,TOP2A,Topoisomerase DNA II alpha 170  kDa,TP2A

Purification: Affinity purification

Immunogen: Synthesized peptide derived from the N-terminal region of human Topo IIalpha

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:20000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide,0.5 % BSA and 50 % glycerol  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details

Target: Topo IIalpha

Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Molecular Weight: 174kDa

Gene ID: 7153

UniProt: P11388