B4GALT1 antibody

Catalog No. ABIN5968159

Product Details anti-B4GALT1 Antibody

Target: B4GALT1 (UDP-Gal:betaGlcNAc beta 1,4 Galactosyltransferase, Polypeptide 1 (B4GALT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This B4GALT1 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Affinity purification

Immunogen: Recombinant protein of human B4GALT1

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for B4GALT1

Target: B4GALT1 (UDP-Gal:betaGlcNAc beta 1,4 Galactosyltransferase, Polypeptide 1 (B4GALT1))

Alternative Name: B4GALT1 (B4GALT1 Products)

Synonyms: B4GAL-T1 antibody, CDG2D antibody, GGTB2 antibody, GT1 antibody, GTB antibody, beta4Gal-T1 antibody, B-1,4-GalT antibody, B-1,4-GalT1 antibody, GalT antibody, Ggtb antibody, Ggtb2 antibody, b4Gal-T1 antibody, beta-1,4-GalT antibody, beta-1,4-GalT1 antibody, beta-1,4-galactosyltransferase 1 antibody, UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 antibody, B4GALT1 antibody, B4galt1 antibody

Background:

Synonyms: 384.Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase,4-galactosyltransferase 1,4-GalTase 1,b4Gal-T1,B4galt1,B4GT1,Beta-1,Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase,Beta4Gal-T1,CDG2D,GGTB2,GT1,GTB,Lactose synthase A protein,N-acetyllactosamine synthase,Nal synthase,Processed beta-1,UDP-Gal:beta-GlcNAc beta-1,UDP-galactose:beta-N-acetylglucosamine beta-1

Background: This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose, all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose <=> UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing. Two transcripts, which differ only at the 5' end, with approximate lengths of 4.1 kb and 3.9 kb encode the same protein. The longer transcript encodes the type II membrane-bound, trans-Golgi resident protein involved in glycoconjugate biosynthesis. The shorter transcript encodes a protein which is cleaved to form the soluble lactose synthase.

Molecular Weight:

Observed_MW: 55kDa

Calculated_MW: 42kDa/43kDa

Gene ID: 2683

UniProt: P15291

Pathways: Glycosaminoglycan Metabolic Process