His Tag antibody

Catalog No. ABIN601531

This Goat Polyclonal antibody specifically detects His Tag in WB, ELISA, IF and IHC.

Quick Overview for His Tag antibody (ABIN601531)

Target: His Tag

Host: Goat

Clonality: Polyclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-His Tag Antibody

Sequence: HHHHHH

Specificity: Recognizes poly-histidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a poly-histidine fusion protein expression vectors.

Purification: Immunoaffinity purified

Immunogen: 6-His peptide (KLH).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (1:100 - 1:500), IHC (1:100 - 1:500), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA. Western Blot: 1-2 μg/mL. ELISA: 0.1-1 μg/mL. Immunohistochemistry: 1:100-1:500. Immunofluorescence: 1:100-1:500.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile ddH2O

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for His Tag

Target: His Tag

Target Type: Tag