TNF alpha antibody (C-Term)

Catalog No. ABIN602484

The Rabbit Polyclonal anti-TNF alpha antibody (ABIN602484) specifically detects TNF alpha in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TNF alpha antibody (C-Term) (ABIN602484)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TNF alpha Antibody

Binding Specificity: C-Term

Specificity: Recognizes human TNFa. Species cross-Reactivity: mouse, rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to the C-terminal of human TNFa.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC (0.5 - 1 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1 μg/mL. Immunohistochemistry: 1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 200 μL sterile ddH20

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % BSA, 0.05 mg Thimerosal, 0.05 mg sodium azide

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF Alpha

Background: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gene ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome