SMAD2 / SMAD3 (pSer423), (pSer425) antibody

Catalog No. ABIN6225575

Product Details

Target: SMAD2 / SMAD3

Binding Specificity: pSer423, pSer425

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Sequence: SSMS

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Phosphorylated Antibodies

Immunogen: A synthetic phosphorylated peptide around S465 & S467 of human Smad2 (NP_005892.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References

Xing, Yu, Zhang, Luo, Lei, Huang, Lin, Huang, Huang, Nong, Zhou, Wei et al.: "Isoviolanthin Extracted from Dendrobium officinale Reverses TGF-β1-Mediated Epithelial⁻Mesenchymal Transition in Hepatocellular Carcinoma Cells via Deactivating the TGF-β/Smad and PI3K/Akt/mTOR ..." in: International journal of molecular sciences, Vol. 19, Issue 6, (2018) (PubMed).

Target Details

Target: SMAD2 / SMAD3

Alternative Name: SMAD2/SMAD3

Background: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012],HSPC193;HsT17436;JV15-2;LDS1C;LDS3;MADH3,Protein phosphorylation,Protein phosphorylation,Protein phosphorylation,SMAD2/SMAD3

Molecular Weight: 48 kDa/52 kDa/25 kDa/35 kDa/43 kDa

Gene ID: 4087, 4088

UniProt: Q15796, P84022