Ku-Holo (p70,83) antibody (CF®647)

Catalog No. ABIN6152929

Product Details

Target: Ku-Holo (p70,83)

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®647

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purpose: Mouse Monoclonal anti-Ku-Holo (p70,83) (KU729), CF647 Conjugate

Characteristics: This antibody recognizes a dimer of two proteins of 70 kDa and ~80 kDa, identified as two subunits of Ku. This MAb This antibody recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Nuclear extract of human HL-60 cells

Clone: KU729

Isotype: IgG1 kappa

Application Details

Application Notes: Immunocytochemistry acetone-fixed cells 0.25-0.5 μg/mL

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Does not react with mouse, rat or chicken, others not known
• Optimal dilution for a specific application should be determined by user

Comment:

Human cancer K562, HL-60, HEp-2 or HeLa cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details

Target: Ku-Holo (p70,83)

Molecular Weight: 70 kDa & 80 kDa

Gene ID: 2547, 7520