Ku-Holo (p70,83) antibody
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- Target
- Ku-Holo (p70,83)
- Reactivity
- Human, Non-Human Primate
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
- Application
- Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Purpose
- Mouse Monoclonal anti-Ku-Holo (p70,83) (KU729), Purified, with BSA
- Characteristics
- This antibody recognizes a dimer of two proteins of 70 kDa and ~80 kDa, identified as two subunits of Ku. This MAb This antibody recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogen
- Nuclear extract of human HL-60 cells
- Clone
- KU729
- Isotype
- IgG1 kappa
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- Application Notes
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Immunocytochemistry acetone-fixed cells 0.25-0.5 μg/mL
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Does not react with mouse, rat or chicken, others not known
- Optimal dilution for a specific application should be determined by user
- Comment
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Human cancer K562, HL-60, HEp-2 or HeLa cells
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 200 μg/mL
- Buffer
- PBS/0.05 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- Ku-Holo (p70,83)
- Molecular Weight
- 70 kDa & 80 kDa
- Gene ID
- 2547, 7520
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