Transglutaminase 2 antibody (CF®568)

Catalog No. ABIN6156774

Product Details anti-Transglutaminase 2 Antibody (CF®568)

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reactivity: Human, Mouse, Rat, Monkey, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transglutaminase 2 antibody is conjugated to CF®568

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Purpose: Mouse Monoclonal anti-Transglutaminase-II (TGM2/419), CF568 Conjugate

Characteristics: This antibody recognizes a 77-85 kDa protein, identified as cellular or tissue transglutaminase II (TGase II). Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD), a genetic, immunologically mediated small bowel enteropathy that causes malabsorption. TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human TGM2 protein

Clone: TGM2-419

Isotype: IgG2a kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

HUVEC Cells. Endothelial cells in placenta, liver, brain, or breast carcinoma. Smooth muscle cells of any origin (e.g. intestine)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Transglutaminase 2

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Alternative Name: Transglutaminase-II (TGM2 Products)

Synonyms: G-ALPHA-h antibody, GNAH antibody, TG2 antibody, TGC antibody, tgm2 antibody, zgc:101081 antibody, TgaseII antibody, TGM2 antibody, fc12b04 antibody, wu:fc12b04 antibody, zgc:136700 antibody, TGL2 antibody, G[a]h antibody, TGase2 antibody, tTG antibody, tTGas antibody, transglutaminase 2 antibody, transglutaminase 2, C polypeptide A antibody, transglutaminase 2b antibody, transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) antibody, transglutaminase 2, C polypeptide antibody, TGM2 antibody, tgm2a antibody, Tgm2 antibody, tgm2b antibody

Molecular Weight: 77-85 kDa

Gene ID: 7052, 517033

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling