Recombinant Histone H1 antibody (CF®647)
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- Target See all Histone H1 Antibodies
- Histone H1
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This Histone H1 antibody is conjugated to CF®647
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)
- Purpose
- Recombinant Rabbit Monoclonal anti-Histone H1 (HH1/1784R), CF647 Conjugate
- Characteristics
- Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant full-length human Histone H1 protein
- Clone
- HH1-1784R
- Isotype
- IgG kappa
- Top Product
- Discover our top product Histone H1 Primary Antibody
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- Application Notes
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- Histone H1
- Abstract
- Histone H1 Products
- Synonyms
- H10 antibody, H1FV antibody, H1 histone family member 0 antibody, H1F0 antibody
- Molecular Weight
- ~30 kDa
- Gene ID
- 3005
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