Integrin beta 4 antibody (CF®568)
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- Target See all Integrin beta 4 (ITGB4) Antibodies
- Integrin beta 4 (ITGB4)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Integrin beta 4 antibody is conjugated to CF®568
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Blocking Reagent (BR)
- Purpose
- Mouse Monoclonal anti-CD104 (UM-A9), CF568 Conjugate
- Characteristics
- This antibody recognizes a protein of 205 kDa, which is identified as integrin beta-4 (ITGB4). Its epitope is localized in the extracellular domain of ITGB4 protein. Integrins are heterodimers comprised of alpha and beta subunits, that are non-covalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta-4 subunit, a receptor for the laminins. This subunit tends to associate with alpha-6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Human squamous cell carcinoma (UM-SCC1)
- Clone
- UM-A9
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product ITGB4 Primary Antibody
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- Application Notes
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Biological blockade partially blocks laminin binding
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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A431 cells, squamous cell carcinoma, colon or placenta
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- Integrin beta 4 (ITGB4)
- Alternative Name
- CD104 (ITGB4 Products)
- Synonyms
- AA407042 antibody, C230078O20 antibody, CD104 antibody, integrin subunit beta 4 antibody, integrin beta 4 antibody, ITGB4 antibody, Itgb4 antibody
- Background
- GP150, Integrin beta-4, ITGB4
- Molecular Weight
- 205 kDa
- Gene ID
- 3691, 632226
- UniProt
- P16144
- Pathways
- Integrin Complex
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