Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) antibody (CF®488A)
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- Target See all Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) Antibodies
- Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)
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Binding Specificity
- AA 356-475
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Reactivity
- Human, Mouse, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- CF®488A
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
- Purpose
- Mouse Monoclonal anti-Spectrin-beta-III (SPTBN2/1584), CF488A Conjugate
- Characteristics
- Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin ? and spectrin ? are present in erythrocytes, whereas spectrin ? II (also designated fodrin ?) and spectrin ? I (also designated fodrin ?) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin ? III is highly homologous to both spectrin ? I and spectrin ? II. Spectrin ? III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human SPTBN2 fragment (aa356-475) (exact sequence is proprietary)
- Clone
- SPTBN2-1584
- Isotype
- IgG1
- Top Product
- Discover our top product SPTBN2 Primary Antibody
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- Application Notes
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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HeLa cells. Pancreas or Liver.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)
- Alternative Name
- Spectrin-beta-III (SPTBN2 Products)
- Synonyms
- Spnb3 antibody, mKIAA0302 antibody, GTRAP41 antibody, SCA5 antibody, spectrin beta, non-erythrocytic 2 antibody, spectrin, beta, non-erythrocytic 2 antibody, Sptbn2 antibody, SPTBN2 antibody
- Background
- Beta III spectrin, SCA5, Spectrin beta chain brain 2, Spectrin beta non-erythrocytic 2, Spectrin non-erythroid beta chain 2, Spinocerebellar ataxia 5, SPTBN2
- Molecular Weight
- 246 kDa
- Gene ID
- 6712, 26915
- UniProt
- O15020
- Pathways
- Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis
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