Interferon gamma antibody (CF®640R)
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- Target See all Interferon gamma (IFNG) Antibodies
- Interferon gamma (IFNG)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Interferon gamma antibody is conjugated to CF®640R
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Application
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- Purpose
- Mouse Monoclonal anti-Interferon gamma (IFNG/466), CF640R Conjugate
- Characteristics
- This antibody recognizes a protein of 20-25 kDa, identified as human interferon. This MAb is specific to human IFN-γ and recognizes both recombinant and native human IFN-gamma. T lymphocytes and NK cells mainly produce IFN-γ. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak anti-viral and anti-proliferative activity, and potentiates the antiviral and anti-tumor effects of IFN-γ (type I interferon). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Purified recombinant human IFN-gamma
- Clone
- IFNG-466
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product IFNG Primary Antibody
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- Application Notes
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Immunohistology (formalin) 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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PMA stimulated human peripheral blood mononuclear cells. Renal or testicular carcinoma.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- Interferon gamma (IFNG)
- Alternative Name
- Interferon gamma (IFNG Products)
- Synonyms
- IFG antibody, IFI antibody, IFN-g antibody, Ifg antibody, IFNG2 antibody, IFN-gamma antibody, IFN-G antibody, IFNG antibody, IFNgamma antibody, TCRalpha antibody, INF-G antibody, ifng antibody, interferon gamma antibody, interferon, gamma 1-2 antibody, IFNG antibody, Ifng antibody, ifng1-2 antibody
- Molecular Weight
- 20-25 kDa
- Gene ID
- 3458, 856
- UniProt
- P01579
- Pathways
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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