Involucrin antibody (CF®405S)
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- Target See all Involucrin (IVL) Antibodies
- Involucrin (IVL)
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Reactivity
- Human, Pig, Dog, Gorilla, Monkey, Owl
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Involucrin antibody is conjugated to CF®405S
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-Involucrin (SY5), CF405S Conjugate
- Characteristics
- This antibody recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Purified involucrin from human keratinocytes
- Clone
- SY5
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product IVL Primary Antibody
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- Application Notes
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Immunohistology formalin-fixed 0.1-0.2 μg/mL
- Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37C, enzyme digestion is better than citrate buffer based epitope unmasking
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence
- Does not react with mouse, others not known
- Optimal dilution for a specific application should be determined by user
- Comment
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MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- Involucrin (IVL)
- Alternative Name
- Involucrin (IVL Products)
- Synonyms
- INV antibody, NPH2 antibody, NPHP2 antibody, 1110019C06Rik antibody, IVL antibody, Nphp2 antibody, inv antibody, involucrin antibody, inversin antibody, IVL antibody, INVS antibody, Ivl antibody, Invs antibody
- Background
- IVL
- Molecular Weight
- 66-170 kDa
- Gene ID
- 3713, 516439
- UniProt
- P07476
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