Thymidine Phosphorylase antibody (CF®640R)
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- Target See all Thymidine Phosphorylase (TYMP) Antibodies
- Thymidine Phosphorylase (TYMP)
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Reactivity
- Human, Rat, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Thymidine Phosphorylase antibody is conjugated to CF®640R
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Purpose
- Mouse Monoclonal anti-Thymidine Phosphorylase / PD-ECGF (P-GF.44C), CF640R Conjugate
- Characteristics
- This antibody recognizes a protein (amino acid 482) of 55 kDa (in vivo 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant full-length human Thymidine Phosphorylase (TP / PD-ECGF) protein
- Clone
- P-GF-44C
- Isotype
- IgG1
- Top Product
- Discover our top product TYMP Primary Antibody
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- Application Notes
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Immunohistology (formalin) 1-2 μg/mL
- Western blotting 0.5-1 μg/mL Immunoprecipitation 0.5-1 μg/500 μg protein lysate
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Optimal dilution for a specific application should be determined by user
- Comment
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HUVEC cells. Breast, Bladder, Lung or Kaposi tumors
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- Thymidine Phosphorylase (TYMP)
- Alternative Name
- Thymidine Phosphorylase / PD-ECGF (TYMP Products)
- Synonyms
- ECGF antibody, ECGF1 antibody, MEDPS1 antibody, MNGIE antibody, MTDPS1 antibody, PDECGF antibody, TP antibody, hPD-ECGF antibody, 2900072D10Rik antibody, Ecgf1 antibody, PD-ECGF antibody, Pdgfec antibody, typh antibody, thymidine phosphorylase antibody, thymidine phosphorylase DeoA antibody, AMP phosphorylase antibody, Thymidine phosphorylase antibody, TYMP antibody, Tymp antibody, deoA antibody, Dd586_0614 antibody, Kvar_4413 antibody, NMAG_RS19330 antibody, Snov_1187 antibody, Fbal_2856 antibody, Ftrac_2071 antibody, Intca_1216 antibody, ARCVE_RS10415 antibody, Sphch_4174 antibody, Sinme_3461 antibody, METOK_RS06355 antibody, Mesop_1831 antibody, typh antibody
- Background
- ECGF, ECGF1, Gliostatin, hPD-ECGF, MEDPS1, MNGIE, MTDPS1, PD-ECGF, PDECGF, Platelet-derived endothelial cell growth factor, TdRPase, Thymidine phosphorylase, TP, Tymp
- Molecular Weight
- 55 kDa
- Gene ID
- 1890, 180903
- UniProt
- P19971
- Pathways
- Signaling Events mediated by VEGFR1 and VEGFR2
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