PSAP antibody (CF®594)

Catalog No. ABIN6165827

Product Details anti-PSAP Antibody (CF®594)

Target: PSAP (Prostate Specific Acid Phosphatase (PSAP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PSAP antibody is conjugated to CF®594

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Mouse Monoclonal anti-Prostate-Specific-Acid Phosphatase (ACPP/1338), CF594 Conjugate

Characteristics: This antibody recognizes a protein of 52 kDa, identified as prostate specific acid phosphatase (PSAP). This enzyme catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. PSAP is found in non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no staining in granulocytes, osteoclasts, parietal cells of the stomach, liver cells, renal cell or breast carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length ACPP protein

Clone: ACPP-1338

Isotype: IgG1 kappa

Application Details

Application Notes: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

PC12 cells or normal prostate or prostate carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for PSAP

Target: PSAP (Prostate Specific Acid Phosphatase (PSAP))

Alternative Name: Prostate-Specific-Acid Phosphatase (PSAP Products)

Background: 5',-nucleotidase (5',-NT), Acid phosphatase prostate, ACP3, Ecto-5',-nucleotidase, Prostatic acid phosphatase (PAP), Prostatic acid phosphatase, Thiamine monophosphatase (TMPase)

Molecular Weight: 52 kDa

Gene ID: 55