CD90 antibody (CF®647)
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- Target See all CD90 (THY1) Antibodies
- CD90 (THY1)
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Reactivity
- Human, Cow
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD90 antibody is conjugated to CF®647
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF)
- Purpose
- Mouse Monoclonal anti-CD90/Thy1 (AF-9), CF647 Conjugate
- Characteristics
- This antibody recognizes a protein of 18-35 kDa, identified as CD90 (also known as Thy1). CD90 is a member of the immunoglobulin superfamily. It may contribute to inhibition of proliferation/differentiation of hematopoietic stem cells and neuron memory formation in the CNS. It consists of a single Ig domain (112 amino acids, 25-35 kDa) inserted into the cell membrane via a GPI anchor. Expressed by hematopoietic stem cells and neurons in all species studied. Its highly expressed in connective tissue and various fibroblast and stromal cell lines, expressed on all thymocytes and peripheral T cells in mice, but in humans expressed only on small % fetal thymocytes, 10-40 % of CD34 cells in bone marrow, and <1 % of CD3 CD4 lymphocytes in peripheral circulation. It is also expressed by human lymph node HEV endothelium but not other endothelia. Lastly, it is expressed by a limited number of lymphoblastoid and leukemic cell lines. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the < a href="https://biotium.com/wp-content/uploads/2013/07/CF-dye-selection-guide.pdf" target="_blank" rel="noopener">CF® Dye Selection Guide for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Human T-lymphoma cells
- Clone
- AF-9
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product THY1 Primary Antibody
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- Application Notes
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Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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IMR-32, CCRF-CEM or MOLT-4 cells. Human uterus.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- CD90 (THY1)
- Alternative Name
- CD90/Thy1 (THY1 Products)
- Synonyms
- CD90 antibody, CD7 antibody, T25 antibody, Thy-1 antibody, Thy-1.2 antibody, Thy1.1 antibody, Thy1.2 antibody, Thy-1 cell surface antigen antibody, thymus cell antigen 1, theta antibody, THY1 antibody, Thy1 antibody
- Background
- CD54, Cell surface glycoprotein P3.58, Human rhinovirus receptor, ICAM-1, Intercellular adhesion molecule 1, Ly 47, Major group rhinovirus receptor, MALA2, MyD10, Surface antigen of activated B cells
- Molecular Weight
- 18-35 kDa
- Gene ID
- 7070, 644697
- UniProt
- P04216
- Pathways
- Cell-Cell Junction Organization
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