Podoplanin antibody (AA 24-126)

Catalog No. ABIN6167017

Product Details anti-Podoplanin Antibody

Target: Podoplanin (PDPN)

Binding Specificity: AA 24-126

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Podoplanin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: Mouse Monoclonal anti-Podoplanin (PDPN/1433), Purified, with BSA

Characteristics: This antibody recognizes a muco-protein of 38-43 kDa, which is identified Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Recombinant human Podoplanin (PDPN) protein fragment (aa24-126) (exact sequence is proprietary)

Clone: PDPN-1433

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL Western blotting 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa Cells. Angiosarcoma or Mesothelioma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: Podoplanin (PDPN Products)

Synonyms: PDPN antibody, AGGRUS antibody, GP36 antibody, GP40 antibody, Gp38 antibody, HT1A-1 antibody, OTS8 antibody, PA2.26 antibody, T1A antibody, T1A-2 antibody, OTS-8 antibody, RANDAM-2 antibody, T1-alpha antibody, T1a antibody, T1alpha antibody, E11 antibody, RTI40 antibody, podoplanin antibody, PDPN antibody, Pdpn antibody

Molecular Weight: 38-43 kDa

Gene ID: 10630, 468675

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport