s100a4 antibody (CF®594)
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- Target See all s100a4 (S100A4) Antibodies
- s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))
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Reactivity
- Human, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This s100a4 antibody is conjugated to CF®594
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- Purpose
- Mouse Monoclonal anti-S100A4 (S100A4/1481), CF594 Conjugate
- Characteristics
- S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, ?6?4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human S100A4 protein
- Clone
- S100A4-1481
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product S100A4 Primary Antibody
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- Application Notes
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Immunohistology (formalin) 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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HeLa, A549 or A375 Cells. Placenta
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))
- Alternative Name
- S100A4 (S100A4 Products)
- Synonyms
- 18A2 antibody, 42A antibody, CAPL antibody, FSP1 antibody, MTS1 antibody, P9KA antibody, PEL98 antibody, 42a antibody, Capl antibody, FSp1 antibody, Mts1 antibody, PeL98 antibody, metastasin antibody, pk9a antibody, S100A4 antibody, P9ka antibody, RNP9KA antibody, S100 calcium binding protein A4 antibody, S100 calcium-binding protein A4 antibody, S100A4 antibody, S100a4 antibody
- Molecular Weight
- 10-12 kDa
- Gene ID
- 6275, 654444
- UniProt
- P26447
- Pathways
- S100 Proteins
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