alpha Fetoprotein antibody (CF®640R)
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- Target See all alpha Fetoprotein (AFP) Antibodies
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This alpha Fetoprotein antibody is conjugated to CF®640R
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-AFP (Alpha-Fetoprotein) (C2 + C3 + MBS-12), CF640R Conjugate
- Characteristics
- This antibody recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant full-length human Alpha fetoprotein
- Clone
- C2 C3 MBS-12
- Isotype
- IgG
- Top Product
- Discover our top product AFP Primary Antibody
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- Application Notes
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Immunohistology formalin-fixed 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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HepG2 cells. Fetal liver or hepatocellular carcinoma
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
- Alternative Name
- AFP (AFP Products)
- Synonyms
- FETA antibody, HPAFP antibody, alpha fetoprotein antibody, alpha-fetoprotein antibody, AFP antibody, Afp antibody
- Background
- Alpha fetoglobulin, FETA, HPAFP
- Molecular Weight
- 70 kDa
- Gene ID
- 174, 518808
- UniProt
- P02771
- Pathways
- C21-Steroid Hormone Metabolic Process
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